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Saturday, February 24, 2007

DIFFERENT TECHNIQUES INVOLVED IN BIOTECHNOLOGY

TISSUES CULTURE TECHNIQUE

Ø Totipotency
Ø Embryo Culture
Ø Single Cell Culture
Ø Micropropagation
Ø Haploid Culture
Ø Somaclonal Variation
Ø Suspension Culture
Ø Artificial Seed
Ø Protoplast Culture

CELL FUSION TECHNIQUE

GENE TRANSFER TECHNIQUE

Methods:

Ø Transfer via agrobacterium spp.
Ø Transfer via electroporation
Ø Transfer via polyethylene glycol [PEG]
Ø Transfer via biolistics
Ø Transfer of microinjection
Ø Liposome Mediated Gene Transfer
Ø Transformation using pollen or pollen tube
Ø Incubation of Dry seeds, Embryos, Tissues or Cells in DNA
Ø Transfer by using Ultrasonification

BIOTECHNOLOGY IN AGRICULTURE, HEALTH AND INDUSTRY




Biotechnology research is increasingly contributing to the improved production and propagation of new cultivated varieties of plants that have a direct role in agricultural practices. In relation to nutritional quality, food output and other useful substances. Some of the important aspects of biotechnology which can be categorized under the following heads:

Ø Micropropagation for biomass energy production.

Ø Production of disease free, disease resistant, insect resistant, herbicide resistant plants.

Ø Induction & Selection of Mutant.

Ø Production of somatic hybrids.

Ø Production of transgenic plants.

Ø Industrial biotechnology.