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Friday, December 30, 2011

Matrix or Media used in column chromatography


Column chromatography is technique used to separate compounds of interest from the others using a column finely packed with media/matrix as stationary phase.

Matrix used should have certain properties these includes:
1. Mechanical Stability
2. Chemical Stability
3. Should be chemically inert



Commonly Used Matrices

Cellulose Based :
Eg: Cellufine

Dextran Based :
Eg: Sephadex, Sephacryl

Agarose Based :
Eg: Sepharose, Biogel A

Polyacrylamide Based :
Eg: Biogel - P

Polystyrene Based :
Eg: Dowex - 50

Silica Based :