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Wednesday, January 2, 2013

Conversion and Calculations of Nucleic Acids and Proteins

Molecular Weights (MW)

Average MW RNA nucleotide = 340 Daltons
MW ss RNA = (# nucleotides) x 340

Average MW RNA basepair = 680 Daltons
MW ds RNA = (# nucleotides) x 680

Average MW DNA nucleotide = 330 Daltons
MW ss DNA = (# nucleotides) x 330

Average MW DNA basepair = 660 Daltons
MW ds DNA = (# basepairs) x 660

Average MW of an amino acid = 110 Daltons
Average MW of a protein = (# of amino acids) x 110

Conversions for Protein and DNA Size and Weight

Average molecular weight of an amino acid = 110 Daltons

333 amino acids = 37 kDa protein

10 kDa protein = 270 bp DNA

100 pmol of 1 kDa protein = 100 ng

1 kb of ds DNA = 6.6 x 10/5 Daltons

1 kb of ss DNA = 3.3 x 10/5 Daltons

1 kb of ss RNA = 3.4 x 10/5 Daltons

Average weight of a nucleotide = 330 Daltons

1 µg of 1000 bp DNA = 1.53 pmol (3.03 pmol of DNA ends)

1 pmol of 1000 bp DNA = 0,66 µg

1 A260 unit of ds DNA = 50 µg/ml

1 A260 unit of ss DNA = 33 µg/ml

1 A260 unit of ss RNA = 40 µg/ml

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