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Thursday, July 18, 2013

A Brief note on Chromatographic Process Scale Up

To get the same results as in lab scale these following parameters need to be kept constant:

Chromatography process scale up

  • Column Length
  • Linear Flow Rate
  • Ratio Between Gradient and Column Length
Constant Column Length

scale up of chromatography column

Desired Capacity should be proportional to the cross sectional area of the column, because same proportion of the column should be occupied by the samples on both the scales


gradient elution

Gradient volume is decided when the diameter of the column is set. Gradient elution in process scale will take the same time as in laboratory scale.

Sometimes a lower flow rate will compensate the decrease in resolution due to ineffective solvent distribution.