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Monday, March 2, 2015

How to analyze Primer Sequence or Oligonucleotide sequence designed for PCR / qPCR


Once a primer is designed for PCR or qPCR, it is important to analyze its properties like melting temperature (Tm), GC content, etc for efficient performance in PCR. Using IDT oligoanalyzer tool one can vary parameters and and analyze the effect on the input primer.


Sample Sequence : GTA TCT GAT CGT CTT CAC TCC C