TISSUES CULTURE TECHNIQUE
Ø Totipotency
Ø Embryo Culture
Ø Single Cell Culture
Ø Micropropagation
Ø Haploid Culture
Ø Somaclonal Variation
Ø Suspension Culture
Ø Artificial Seed
Ø Protoplast Culture
CELL FUSION TECHNIQUE
GENE TRANSFER TECHNIQUE
Methods:
Ø Transfer via agrobacterium spp.
Ø Transfer via electroporation
Ø Transfer via polyethylene glycol [PEG]
Ø Transfer via biolistics
Ø Transfer of microinjection
Ø Liposome Mediated Gene Transfer
Ø Transformation using pollen or pollen tube
Ø Incubation of Dry seeds, Embryos, Tissues or Cells in DNA
Ø Transfer by using Ultrasonification
BIOTECHNOLOGY IN AGRICULTURE, HEALTH AND INDUSTRY
Biotechnology research is increasingly contributing to the improved production and propagation of new cultivated varieties of plants that have a direct role in agricultural practices. In relation to nutritional quality, food output and other useful substances. Some of the important aspects of biotechnology which can be categorized under the following heads:
Ø Micropropagation for biomass energy production.
Ø Production of disease free, disease resistant, insect resistant, herbicide resistant plants.
Ø Induction & Selection of Mutant.
Ø Production of somatic hybrids.
Ø Production of transgenic plants.
Ø Industrial biotechnology.
Ø Totipotency
Ø Embryo Culture
Ø Single Cell Culture
Ø Micropropagation
Ø Haploid Culture
Ø Somaclonal Variation
Ø Suspension Culture
Ø Artificial Seed
Ø Protoplast Culture
CELL FUSION TECHNIQUE
GENE TRANSFER TECHNIQUE
Methods:
Ø Transfer via agrobacterium spp.
Ø Transfer via electroporation
Ø Transfer via polyethylene glycol [PEG]
Ø Transfer via biolistics
Ø Transfer of microinjection
Ø Liposome Mediated Gene Transfer
Ø Transformation using pollen or pollen tube
Ø Incubation of Dry seeds, Embryos, Tissues or Cells in DNA
Ø Transfer by using Ultrasonification
BIOTECHNOLOGY IN AGRICULTURE, HEALTH AND INDUSTRY
Biotechnology research is increasingly contributing to the improved production and propagation of new cultivated varieties of plants that have a direct role in agricultural practices. In relation to nutritional quality, food output and other useful substances. Some of the important aspects of biotechnology which can be categorized under the following heads:
Ø Micropropagation for biomass energy production.
Ø Production of disease free, disease resistant, insect resistant, herbicide resistant plants.
Ø Induction & Selection of Mutant.
Ø Production of somatic hybrids.
Ø Production of transgenic plants.
Ø Industrial biotechnology.